Rate of Enzyme Reaction on Substrate Lab Report

Title of Your Report Be a little creative “Bio366L Lab Report” gets boring Name Bio366L – Section # Group # Date TA: Name of TA Introduction – 5 points This document shows you exactly how I want the lab reports formatted. You should try to follow this layout as best as you can. I have included mock figures, tables and formulas to show you what I am looking for when grading your data. Sticking to specific formats and page lengths are an important part of publishing scientific data. In fact, most journals will not accept your work if it is too long or formatted incorrectly. For this reason, sticking to the format and page length is worth 2 point on your total lab report grade.

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Grammar and spelling are also important for effective communication. Since modern word processing software checks for both, please use these to make sure your report is not full of errors. I will subtract up to 5 points off your total report grade for bad grammar and spelling. The format should be double spaced text in 12-point font with 1-inch margin all around. I recommend using a serif font (like Cambria or Times), but I know you may have a limited selection of fonts so any 12-point font that is legible is acceptable. You should have a section title that is bold and in 18-point font before each section, just like the one that says ‘Introduction’ here. You should have a title page (which doesn’t count against your page limit), where you also list the names of your collaborators (the other people in your lab group if any). Your title page with collaborator list (including yourself) is worth 1 point. The page requirement for the Lab Report is 5 to 7 pages. Remember, the title page and your list of references DO NOT count against your page limit. You may also have additional appendices at the end of your report which do not count toward your page limit as well. Your introduction should probably be around 1 – 1.5 pages in length. Here, you are demonstrating you having a firm grasp on the background information of your topic of choice. Without showing that you have command over sufficient knowledge first, the rest of your report would not elicit trust in your reader. You could summarize IN YOUR OWN WORDS, the results of your literature research. This is one of the best places to get in your references to primary literature too. Wikipedia might be a good place to start your initial search, but actually comb through the references listed in the Wikipedia article. Some of the reference listed could be primary sources that you can use. Do not list the Wikipedia article itself as a reference. Within your introduction, you should also state the purpose of your paper, such as 1) elucidating the properties of something, 2) detailing the process of a procedure, 3) demonstrating a concept, etc.

You should clearly describe how the experiments you are about to discuss in detail will demonstrate the concepts you just wrote about. This does not need to be in-depth, but you should have two or three sentences devoted to this. The introduction is worth 5 points in total for your first report: ● 3 points for background info about the experiments (some from primary literature). History of cloning, the various techniques ● 1 point for referencing your research adequately (your lab manual should be the bare minimum reference here). ● 1 1 point for stating the purpose of this whole experiment. Materials and Methods – 3 points This section is only worth 3 points because you can state that you followed the lab manual, which pages you used, and reference it as a source (just like you did before). If you made modifications to any step in the procedure, list those as well. In addition, this section should be written with the mindset that if another person were to repeat your experiment, he/she should get the same results as you did. Results – 5 points In your materials and methods, you should have listed the various experiments and test methods you have performed. Here is where you provide the findings of each experiment. Present the data as they are without bias and undue interpretation. Be conservative in your data presentation, and only show what you need to in order to get your point across. Overwhelming amounts of poorly formatted data are not only confusing; they will increase your page length and ultimately cost you points. Please pay attention to how I want your data formatted. You must label AND caption every figure, table and/or formula. This is worth 1 point. If you need to, you may combine the Figure title and caption together, but you must have a narrative accompany each figure and also reference your figure in your text; instead of having a figure just floating in your report without a purpose. 2 Conclusions – 10 points Here is where you get the majority of your points for the lab report. First, introduce each experiment (part A, B, C…etc.) by discussing the objective of performing it (what are you trying to show), and talk briefly about how you obtained the data (DO NOT reiterate the methods, just describe the basic concept). This is worth 2 points. You do not need to include every piece of data you obtained. What’s most important here is to show that you performed an experiment and obtained data that either do or do not support the objective of said experiment. If you used data that your lab group did not generate, you must reference your data source, and explain why you did not use your own data. This is a good opportunity to demonstrate your understanding of the experiment by describing what went wrong with your procedure. Your discussion of the data is worth 3 points. You stated your data as they are without bias in your results section, now is the time for you to present your interpretation/analysis. What do all the data suggest? Synthesize a conclusion using all the available data (including data from your background research). It is Ok if you have conflicting data. State where you think the source of conflict might be and offer solutions. If your data are unclear, then propose other experiments you might do to obtain clarity in your next approach. Are there anything you can do differently next time to save your data from being useless? All of this, plus relating the discussion back to your experimental objective is worth 4 points. You should specifically state the questions asked in the lab manual at the end of each experiment, and answer those questions by referencing your data and conclusions for that experiment. This is worth 1 point. I expect to have a concise discussion of objectives, data, conclusions, and answers to the lab manual questions (if any) for each experiment BEFORE you move on to discussing the next one. This will make it much easier for me to grade each part of the exercise independently, so if you missed a day or something went wrong with the next part, I can take that into account separately from the ‘good’ data. Heading 1 Data title 1 Data title 2 3 Heading 2 Heading 3 Heading 4 56.7 67.8 78.9 12.3 34.5 67.8 References – 2 points This should be some easy points. You get 1 point for having AT LEAST 3 references, and 1 point for having AT LEAST one primary literature reference. Primary literature means original, not previously published research (reviews of lots of other people’s research don’t count). You can find these by going to PubMed, Google Scholar, or using the library research tools. You may use any reference style, but keep in mind the page limits. I recommend the APA citation style or the number-in-line style, where you cite using a superscript number. 1 You will have to list your

Rate of Enzyme Reaction on Substrate Lab Report